Journal: Cellular & Molecular Biology Letters
Article Title: Monitoring correlates of SARS-CoV-2 infection in cell culture using a two-photon-active calcium-sensitive dye
doi: 10.1186/s11658-024-00619-0
Figure Lengend Snippet: Automatic analysis of the 2P microscopy images of infected cells using SARS-CoV-2 variants D614G and B.1.1.7. A Purple contours indicate the areas automatically defined as particles during this analysis. B , C , E , F Spider charts showing the effects of B variant, C virus titer, E dye concentration, and F photomultiplier tube (PMT) relative voltage on the range-normalized values of seven different image parameters obtained from 2P microscopy images. The error bars for the spider charts can be found in Supplementary Fig. 7. D The image parameter called “relative signal area” shows a significant increase at virus titers higher than TCID 50 10 –3 mL −1 . G The image parameter called “image mean intensity” for the two studied variants at high virus titer (TCID 50 > 10 –3 mL −1 ) at 0.05 μM and 0.5 μM dye concentration. H t -Distributed stochastic neighbor embedding (t-SNE) 2D plot obtained from all the seven image parameters recorded at 0.5 µM dye concentration shows three clusters depending on the virus variant and titer. Inset shows that the three groups, namely no or low infection, infection with D614G variant, and infection with B.1.1.7 variant are clearly separated along the first dimension. I Classification of the images corresponding to different variants at various virus titer in three clusters by seven-dimensional Gaussian mixture model clustering (error bars show standard deviation; significance levels as * p ≤ 0.1; ** p ≤ 0.05; *** p ≤ 0.01.)
Article Snippet: Seven parameters characterizing the detected particles on the images were defined for each image: (i) relative signal area, (ii) image mean intensity, (iii) mean of the threshold area, (iv) maximum particle intensity, (v) average particle size, (vi) particle percentage area, and (vii) particle mean intensity. t -Distributed stochastic neighbor embedding (t-SNE) 2D plot was obtained in MATLAB using the built-in “tsne” function with default random number generation, a perplexity value of 10, and exaggeration value of 50 on all seven parameters of images acquired with 0.5 μM dye concentration (Supplementary Fig. 12).
Techniques: Microscopy, Infection, Variant Assay, Virus, Concentration Assay, Standard Deviation
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